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TRIALS
RESULTS
(1)
The following trials were independently undertaken by the Institute for Animal Health.
Study Title
An in vitro study to determine the optimum concentration of
Bi-OO-Cyst and the most effective exposure time to prevent sporulation of Eimeria tenella OOcysts |
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At present there is no product on the market which is effective against
Eimeria that has passed the statutory tests for disinfectants.
The effectiveness of killing Eimeria spp. by a disinfectant can be
determined by its ability to interfere with the process of OOcyst
sporulation. The study was conducted in two stages. During Stage 1, a
range of disinfectant concentrations and exposure times were
investigated. Concentrations and exposures which were most effective
were then investigated further in Stage 2.
Stage 1 utilised a factorial design, with no replication, to investigate a
range of exposure times and dilutions.
In Stage 2, exposure of OOcysts to
Bi-OO-cyst for 120 minutes at
1/30 and 1/50 dilutions was investigated further, this time with
replicated samples.
The effectiveness of a 1/125 dilution following exposure for 120, 180
and 240 minutes was also investigated, together with overnight
exposure to a 1/30 dilution. |
Stage 1 Results
Exposure of
unsporulated oocysts to 1/30 or 1/50 dilutions of the
Bi-OO-cyst for
90 or 120 minutes resulted in abnormal morphological changes in
some of the OOcysts following sporulation, suggesting that they were
no longer viable.
This effect was most pronounced following 120 minutes exposure to a 1/30
dilution of the product where over 96% of the OOcysts were deemed
“irregular”.
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This effect was most pronounced following 120 minutes exposure to a
1/30 dilution of the product where over 96% of the OOcysts were deemed
"irregular".
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Stage 2 Results
After two hours, 96% of
OOcysts failed to sporulate at 1:30 |
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After an overnight exposure
at 1:30 (in excess of four hours), there was no viable sporulation of
OOcysts, showing that Bi-00-cyst is an effective and powerful coccidacidal
disinfectant. |
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In vivo
Trials
Two groups of birds, each of six animals. One group was infected
with OOcysts that had been exposed to disinfectant prior to
sporulation and a control group was infected with OOcysts which were
simply exposed to water.
Faeces were collected from the birds on the fifth to eighth day
following infection, which is when we expected the OOcyst counts to
peak. The total number of OOcysts in these bulked samples was then
calculated. Hence we obtained two counts for each bird.
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In vivo Trial Results
The two groups
of birds demonstrated the power of
Bi-OO-Cyst to combat the
pathogen burden.
Bi-OO-Cyst considerably reduced the OOCyst challenge.
Future in vivo trials to investigate a range of exposure times
for sporulated OOCysts will provide further data on the
disinfectants versatility and efficacy. |
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| Bi-OO-Cyst Test Results (2) Bi.00.2978 Test Data Recommendation of use Powerpoint Presentation |
