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Bi.00.2978
Uses
Bi.00.2978
is for the formulation of disinfectants for use in hospitals, surgeries,
industry, institutions, stables and all livestock housing. For the
formulation of all-purpose disinfectants and disinfectant liquid soaps.
Chemical and Physical Data
Bi.00.2978/2978.Na
Compatibility/behaviour:
As
with other groups of active ingredients, the microbicidal effectiveness
of Bi.00.2978/2978.Na can be affected by the formulating components of
the disinfectant, the pH of the ready-to-use dilutions, the degree of
contamination/bacterial count, the amount of dirt/protein, the hardness of
the water and the temperature. As a phenolic active ingredient,
Bi.00.2978 is generally preferable for heavy-duty disinfection.
Bi.00.2978 comes into its own wherever there is a high risk of
infection, strong organic impurities are present or whenever water with
a high hardness is used.
pH:
Bi.00.2978
works best in an acidic, neutral or weakly basic environment.
Bi.00.2978/2978.Na is, however, stable over a wide pH range from 1 to
14. For this reason, it is also possible to formulate alkaline
disinfectants, although the application concentrations of Bi.00.2978
would have to be somewhat higher in this case. Alkaline disinfectants
are characterised by their rapid, strong dirt-dissolving power:
disinfection and cleaning are possible in one step. For such
agents we recommend the direct use of Bi.00.2978 or the formulation of
an aqueous alkaline solution of Bi.00.2978 that is miscible with water
in any ratio.
Disinfectant effect:
The dependence of Bi.00.2978 efficacy
on factors such as the pH of the ready-to-use dilution, degree of
contamination/micro-organism count, water hardness and dirt content has been
investigated on a standard formulation"SF-2978" (see Table II) as an
example. The results of these tests are given in Tables III and IV.
The dependency of the bacterial count of
the microbicidal effectiveness of Bi.00.2978 is low in the case of Escherichia coli and somewhat more marked in the case of
Staphylococcus aureus and Pseudomonas aeruginosa. There are
no particularly significant differences in effectiveness when the
preparations are used in water of standard hardness is used,
effectiveness data for “SF-2978” is less in the alkaline range (pH 8.0)
than in the acidic range of pH 4.0, both without and with serum. These
factors should be taken into account when using disinfectants. Higher
application concentrations are required for disinfectant measures that
meet particular standards.
Bi.00.2978
Spectrum of activity – minimum inhibitory
concentrations (MICs)
Table I
Lists the minimum inhibitory
concentrations (MIC) in mg/l of Bi.00.2978 on nutrient agar contaminated
with various micro-organisms.
| Bacteria |
| Aeromonas
punctata |
200 |
Mycobacterium terrae DSM 43227 |
100 |
| Bacillus
mycoides |
100 - 150 |
Propionibacterium acnes DSM 20458 |
100 - 200 |
| Bacillus
subtilis |
150 |
Proteus
mirabilis |
500 |
|
Desulfovibrio desulfuricans |
35 |
Pseudomonas
aeruginosa |
500 - 800 |
|
Enterobacter aerogenes |
200 |
Pseudomonas fluorescens |
500 |
| Escherichia
coli |
200 - 250 |
Salmonella
choleraesuis DSM 4224 |
200 |
| Escherichia
coli EHEC DSM 8579 |
200 |
Staphylococcus aureus MRSA DSM 2569 |
100 - 200 |
| Leuconostoc
mesenteroides |
200 |
Staphylococcus aureus MRSA clinical isolate |
200 |
| Listeria
monocylogenes DSM 20600 |
100 - 200 |
|
|
| |
|
|
|
| Yeast |
| Candida albicans |
200 |
Saccharomyces bailii |
200 |
| Candida
krusei |
200 |
Rhodotorula
rubra |
100 |
| Rhodotorula
mucilaginosa |
100 |
Torula
utilis |
200 |
|
Saccharomyces cerevisiae |
200 |
|
|
| |
|
|
|
| Mould
fungi |
| Alternaria
tenuis |
75 - 100 |
Microsporium canis CBS 38564 |
10 - 100 |
| Aspergillus
flavus |
100 |
Mucor
racemosus |
200 |
| Aspergillus
niger |
100 |
Penicillium
brevicaule |
100 - 200 |
| Aspergillus
terreus |
200 |
Rhizopus
stolonifer |
200 |
| Aspergillus
ustus |
200 |
Trichophyton mentagrophytes CBS 26379 |
100 |
| Chaetomium
globosum |
75 - 100 |
Trichophyton rubrum DSM 4167 |
10 - 100 |
The disinfectants properties can be
optimised through the deliberate combination of various disinfectant
active ingredients with suitable formulating auxiliaries such as
surfactants, solvents, sequestering agents, fragrances, corrosion
inhibitors, etc.
Composition of the standard
formulation "SF-2978":
10 % Bi.00.2978
25 % Mersolat W 93 (sec. alkane
sulphonate)
20 % isopropanol
45 % demineralised water
Table II
Lists the minimum
inhibitory concentrations (MIC) in mg/l of the guide formulation
“SF-2978” on nutrient agar contaminated with various micro-organisms.
| Test
organism |
mg / l |
| Escherichia
coli (EHEC) DSM 8579 |
2000 |
| Listeria
monocytogenes DSM 20600 |
1000 |
| Mycobacterium
terrae ATCC 15755 |
2000 |
|
Propionibacterium acnes DSM 20458 |
2000 |
|
Staphylococcus aureus MRSA DSM 2569 |
1000 |
|
Staphylococcus aureus MRSA Feldstamm |
1000 |
| Trichophyton
mentagrophytes CBS 26379 |
1000 |
| Trichophyton
rubrum DSM 4167 |
1000 |
| Microsporium
canis CBS 38564 |
1000 |
Serum loading
was effected using 10 % albumin and 1% yeast extract. To determine the
compatibility with hard water, measurements were carried out using water
of standardised hardness (WSH, 17º hardness = 300 ppm Ca CO3)
and completely demineralised water (DW).
Table III
indicates the bactericidal effect as a function of the pH, with and
without protein loading.
Table IV indicates the bactericidal
effect as a function of the bactericidal count and the water hardness.
Table III
Bactericidal effect of Bi.00.2978,
tested as a standard formulation “SF-2978” in accordance with DGHM
guidelines for qualitative suspension disinfection.
-
Dependence on pH, with and without protein
loading
-
High bacterial contamination: approx. 107
bacteria per ml disinfectant solution
|
Test organism |
Contact time (mins) |
Lethal active concentration in ppm
Bi.00.2978 |
|
WSH, pH4
|
WSH,
pH 8 |
|
without serum |
with
serum |
without serum |
with serum |
|
Staphylococcus aureus |
5 – 60 |
500 – 75 |
7500 |
1000 – 500 |
5000 – 2500 |
|
Escherichia coli |
5 – 60 |
1000 – 250 |
1000 – 500 |
1000 – 500 |
2500 – 500 |
|
Pseudomonas aeruginosa |
5
– 60 |
500 – 75 |
1000 – 5000 |
1000 – 750 |
2500 – 1000 |
Table
IV
Bactericidal effect of Bi.00.2978,
tested as a standard formulation"SF-29782" in accordance with DGHM
guidelines for qualitative suspension disinfection
-
Dependence on the bacteria count, approx. 107
or 103 bacteria per ml disinfectant, with and without protein
loading
-
Two different degrees of water hardness
|
Test organism |
Contact time (mins) |
Bacteria / ml |
Lethal active concentration in
ppm Bi.00.2978 |
|
WSH* |
DW** |
|
without serum |
with
serum |
without serum |
with serum |
|
Staphylococcus aureus |
5 – 60 |
approx. 107 |
750 - 500 |
2500 |
1000 – 750 |
2500 - 1000 |
|
approx.
103 |
250 – 100 |
1000 – 750 |
500 – 250 |
2500 – 1000 |
|
Escherichia coli |
5 – 60 |
approx 107 |
750 - 500 |
2500 – 750 |
1000 – 500 |
1000 - 750 |
| approx 103 |
500 |
1000 – 750 |
1000 – 500 |
1000 - 500 |
|
Pseudomonas aeruginosa |
5 – 60 |
approx 107 |
1000 – 500 |
2500 – 750 |
2500 – 500 |
2500 – 750 |
| approx 103 |
500 |
1000 – 750 |
500 – 250 |
1000 – 750 |
*WSH = water of standardised hardness,
300 ppm CaCO3
**DW =
demineralised water
Bi.00.2978 R-classes
R 21/22, R 41, R 43.
R50
Bi.00.2978.Na R-classes
R 21/22, R 34
Registration / Approval /
Recommendations
USA:
FDA: In the
USA the active ingredient Bi.00.2978 is approved for use as a
preservative for food contact applications.
EPA: Bi.00.2978 Preservative is
approved for use in glues, adhesives, leather, latex emulsions and
metal-working fluids.
For use in the
manufacture of disinfectants, the EPA Reg. No. is 39967-8
Further up-to-date information on the
status of registration of our products can be obtained from:
The Administration Director
BioLink Systems Limited
Meadow View
Norwich
GB-NR5 0LA
The current safety data sheet should
be observed. This contains further information on labelling, transport
and storage as well as information on handling, product safety, toxicity
and ecology.
Use biocides safely. Always read
the label and product information before use.
E&oe
©
BioLink
2004 |
