BioLink - DEFRA approved cleaners and disinfectants

Bi-OO-Cyst

Bi.00.2978

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Uses

Bi.00.2978 is for the formulation of disinfectants for use in hospitals, surgeries, industry, institutions, stables and all livestock housing. For the formulation of all-purpose disinfectants and disinfectant liquid soaps.

Chemical and Physical Data
Bi.00.2978/2978.Na
Compatibility/behaviour:

As with other groups of active ingredients, the microbicidal effectiveness of Bi.00.2978/2978.Na can be affected by the formulating components of the disinfectant, the pH of the ready-to-use dilutions, the degree of contamination/bacterial count, the amount of dirt/protein, the hardness of the water and the temperature. As a phenolic active ingredient, Bi.00.2978 is generally preferable for heavy-duty disinfection. Bi.00.2978 comes into its own wherever there is a high risk of infection, strong organic impurities are present or whenever water with a high hardness is used.

pH:

Bi.00.2978 works best in an acidic, neutral or weakly basic environment. Bi.00.2978/2978.Na is, however, stable over a wide pH range from 1 to 14. For this reason, it is also possible to formulate alkaline disinfectants, although the application concentrations of Bi.00.2978 would have to be somewhat higher in this case. Alkaline disinfectants are characterised by their rapid, strong dirt-dissolving power: disinfection and cleaning are possible in one step. For such agents we recommend the direct use of Bi.00.2978 or the formulation of an aqueous alkaline solution of Bi.00.2978 that is miscible with water in any ratio.

Disinfectant effect:

The dependence of Bi.00.2978 efficacy on factors such as the pH of the ready-to-use dilution, degree of contamination/micro-organism count, water hardness and dirt content has been investigated on a standard formulation"SF-2978" (see Table II) as an example. The results of these tests are given in Tables III and IV.

The dependency of the bacterial count of the microbicidal effectiveness of Bi.00.2978 is low in the case of Escherichia coli and somewhat more marked in the case of Staphylococcus aureus and Pseudomonas aeruginosa. There are no particularly significant differences in effectiveness when the preparations are used in water of standard hardness is used, effectiveness data for “SF-2978” is less in the alkaline range (pH 8.0) than in the acidic range of pH 4.0, both without and with serum. These factors should be taken into account when using disinfectants. Higher application concentrations are required for disinfectant measures that meet particular standards.

Bi.00.2978
Spectrum of activity – minimum inhibitory concentrations (MICs)

Table I

Lists the minimum inhibitory concentrations (MIC) in mg/l of Bi.00.2978 on nutrient agar contaminated with various micro-organisms.

Bacteria
Aeromonas punctata	200	Mycobacterium terrae DSM 43227	100
Bacillus mycoides	100 - 150	Propionibacterium acnes DSM 20458	100 - 200
Bacillus subtilis	150	Proteus mirabilis	500
Desulfovibrio desulfuricans	35	Pseudomonas aeruginosa	500 - 800
Enterobacter aerogenes	200	Pseudomonas fluorescens	500
Escherichia coli	200 - 250	Salmonella choleraesuis DSM 4224	200
Escherichia coli EHEC DSM 8579	200	Staphylococcus aureus MRSA DSM 2569	100 - 200
Leuconostoc mesenteroides	200	Staphylococcus aureus MRSA clinical isolate	200
Listeria monocylogenes DSM 20600	100 - 200

Yeast
Candida albicans	200	Saccharomyces bailii	200
Candida krusei	200	Rhodotorula rubra	100
Rhodotorula mucilaginosa	100	Torula utilis	200
Saccharomyces cerevisiae	200

Mould fungi
Alternaria tenuis	75 - 100	Microsporium canis CBS 38564	10 - 100
Aspergillus flavus	100	Mucor racemosus	200
Aspergillus niger	100	Penicillium brevicaule	100 - 200
Aspergillus terreus	200	Rhizopus stolonifer	200
Aspergillus ustus	200	Trichophyton mentagrophytes CBS 26379	100
Chaetomium globosum	75 - 100	Trichophyton rubrum DSM 4167	10 - 100

The disinfectants properties can be optimised through the deliberate combination of various disinfectant active ingredients with suitable formulating auxiliaries such as surfactants, solvents, sequestering agents, fragrances, corrosion inhibitors, etc.

Composition of the standard formulation "SF-2978":

10 % Bi.00.2978

25 % Mersolat W 93 (sec. alkane sulphonate)

20 % isopropanol

45 % demineralised water

Table II

Lists the minimum inhibitory concentrations (MIC) in mg/l of the guide formulation “SF-2978” on nutrient agar contaminated with various micro-organisms.

Test organism	mg / l
Escherichia coli (EHEC) DSM 8579	2000
Listeria monocytogenes DSM 20600	1000
Mycobacterium terrae ATCC 15755	2000
Propionibacterium acnes DSM 20458	2000
Staphylococcus aureus MRSA DSM 2569	1000
Staphylococcus aureus MRSA Feldstamm	1000
Trichophyton mentagrophytes CBS 26379	1000
Trichophyton rubrum DSM 4167	1000
Microsporium canis CBS 38564	1000

Serum loading was effected using 10 % albumin and 1% yeast extract. To determine the compatibility with hard water, measurements were carried out using water of standardised hardness (WSH, 17º hardness = 300 ppm Ca CO₃) and completely demineralised water (DW).

Table III indicates the bactericidal effect as a function of the pH, with and without protein loading.

Table IV indicates the bactericidal effect as a function of the bactericidal count and the water hardness.

Table III

Bactericidal effect of Bi.00.2978, tested as a standard formulation “SF-2978” in accordance with DGHM guidelines for qualitative suspension disinfection.

Dependence on pH, with and without protein loading
High bacterial contamination: approx. 10⁷ bacteria per ml disinfectant solution

Test organism	Contact time (mins)	Lethal active concentration in ppm Bi.00.2978
		WSH, pH4		WSH, pH 8
		without serum	with serum	without serum	with serum
Staphylococcus aureus	5 – 60	500 – 75	7500	1000 – 500	5000 – 2500
Escherichia coli	5 – 60	1000 – 250	1000 – 500	1000 – 500	2500 – 500
Pseudomonas aeruginosa	5 – 60	500 – 75	1000 – 5000	1000 – 750	2500 – 1000

Table IV

Bactericidal effect of Bi.00.2978, tested as a standard formulation"SF-29782" in accordance with DGHM guidelines for qualitative suspension disinfection

Dependence on the bacteria count, approx. 10⁷ or 10³ bacteria per ml disinfectant, with and without protein loading
Two different degrees of water hardness

Test organism	Contact time (mins)	Bacteria / ml	Lethal active concentration in ppm Bi.00.2978
			WSH*		DW**
			without serum	with serum	without serum	with serum
Staphylococcus aureus	5 – 60	approx. 10⁷	750 - 500	2500	1000 – 750	2500 - 1000
Staphylococcus aureus	5 – 60	approx. 10³	250 – 100	1000 – 750	500 – 250	2500 – 1000
Escherichia coli	5 – 60	approx 10⁷	750 - 500	2500 – 750	1000 – 500	1000 - 750
Escherichia coli	5 – 60	approx 10³	500	1000 – 750	1000 – 500	1000 - 500
Pseudomonas aeruginosa	5 – 60	approx 10⁷	1000 – 500	2500 – 750	2500 – 500	2500 – 750
Pseudomonas aeruginosa	5 – 60	approx 10³	500	1000 – 750	500 – 250	1000 – 750

*WSH = water of standardised hardness, 300 ppm CaCO₃ **DW = demineralised water

Bi.00.2978 R-classes

R 21/22, R 41, R 43. R50

Bi.00.2978.Na R-classes

R 21/22, R 34

Registration / Approval / Recommendations USA:

FDA: In the USA the active ingredient Bi.00.2978 is approved for use as a preservative for food contact applications.

EPA: Bi.00.2978 Preservative is approved for use in glues, adhesives, leather, latex emulsions and metal-working fluids.

For use in the manufacture of disinfectants, the EPA Reg. No. is 39967-8

Further up-to-date information on the status of registration of our products can be obtained from:

The Administration Director
BioLink Systems Limited
Meadow View
Norwich
GB-NR5 0LA

The current safety data sheet should be observed. This contains further information on labelling, transport and storage as well as information on handling, product safety, toxicity and ecology.

Use biocides safely. Always read the label and product information before use.

Bi-OO-Cyst Test Results (1) Test Results (2) Recommendation of use Powerpoint Presentation

BioLink Limited - Unit 2 Becklands Park Industrial Estate, York Road, Market Weighton YO43 3GA
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